DescriptionGenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. Protein G Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg goat IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume5 ml settled resin (10 ml 50% slurry)Storage:Store at 4°C, do NOT freeze. Key Features

• Broad IgG binding spectrum
• Binding specificity complements of protein A
• Agarose media
• No specific albumin binding
• Optimized homogeneous recombinant ligand
• High capacity
• Available in bulk size

Specifications

Ligand	Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand	3
MW of ligand	Approximately 22 kDa
PI of ligand	4.69
Degree of substitution	Approximately 2 mg protein G/ml settled resin
Static binding capacity	>20 mg goat IgG/ml settled resin
Matrix spherical	agarose, 4% cross-linked
Average particle size	90 μm (45-165 μm)
Storage solution	1X PBS containing 20% ethanol
Storage & Stability	18 months if store unopened at 2-8℃