InstaBlue Protein Stain Solution

InstaBlue Protein Stain Solution
Product Code: B8226-1000
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Chemical Properties

Cas No. 6104-58-1 SDF Download SDF
Synonyms Acid Blue 90,CBBG,Coomassie Brilliant Blue G-250,NSC 328382
Chemical Name N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, sodium salt
Canonical SMILES CC1=CC(N(CC)CC2=CC=CC(S(=O)([O-])=O)=C2)=CC=C1/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C\5C)=[N+](CC6=CC=CC(S(=O)([O-])=O)=C6)/CC.[Na+]
Formula C47H48N3O7S2 • Na M.Wt 854
Solubility Soluble in DMSO Storage Store at room temperature for 1 year
Note: The staining solution is a suspension, please be sure to mix it upside and down before use!
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.


Ultra-fast staining – Results in 5 minutes or less.

One-step procedure – No washing, fixing, microwaving or destaining.

High sensitivity – 5ng bands detectable.

Clear background – High signal/noise ratio.

Flexible – No over staining.

Reliable – Quantitative Batch to batch consistency.

Efficient – Only 25ml per gel.

Safe composition – Non-toxic. No fume hood or solvent disposal required.

Methanol free – No gel shrinkage or protein methylation.

Acetic acid free – No protein aceylation.

MS Compatible – No residual methylation or acetylation.


InstaBlue Protein Stain Solution is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique patented mechanism of action stains proteins in 5 minutes while leaving a clear background without the need to fix, wash or destain. InstaBlue Protein Stain Solution is formulated for safe use and easy disposal. 
InstaBlue Protein Stain Solution is very convenient for use and needs only simple one step protocol to finish the rapid coomassie blue staining of protein gels. Some premade and traditional home-made Coomassie R-250 protein stains can take 3 hours or more to fully stain gels.
Because of InstaBlue’s protein specific staining which gives a clear background, improving the signal/noise ratio of the stain, InstaBlue Protein Stain Solution has high sensitivity and can detect protein levels as low as 5 ng per band when staining overnight. 
InstaBlue Protein Stain Solution is non-toxic and thanks to its methanol free composition will not shrink gels. InstaBlue will stain at least 40 mini-gels per 1 L, staining with only 25ml per mini-gel, which allows you to use less protein staining solution. While homemade solutions and some pre-mixed solutions need approximately 50-100ml for staining one gel.


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