CommaXP™ plasmid preparation kits are suited for plasmid miniprep, midiprep and maxiprep from bacterial lysates.
Bacterial cultures are first lysed, and the resulting lysates are applied into spin columns. Under low pH and chaotropic conditions, nucleic acids specifically bind to silica membrane while polysaccharides and proteins pass through.
Impurities are further removed by washing.
Finally, under low-salt conditions, nucleic acids are desorbed and eluted from the membrane.
Plasmids purified by CommaXP™ plasmid preparation kits are suitable for various downstream routine applications, including restriction digestion, PCR amplification, sequencing, ligation and transformation.