• Protein A Resin
DescriptionGenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume5 ml settled resin (10 ml 50% slurry)Storage:Store at 4°C, do NOT freeze. Key Features
  • High IgG-binding capacity
  • Resin is reusable up to 10 times with no significant loss of binding capacity.
Characteristics:
Ligand Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand 5
MW of ligand Approximately 34 kDa
PI of ligand 5.17
Degree of substitution Approximately 2 mg protein A/ml settled resin
Static binding capacity >20 mg human IgG/ml settled resin
Matrix sphericalagarose, 4%
Average particle size90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months when stored unopened at 2-8℃

Write a review

Note: HTML is not translated!
    Bad           Good
Captcha

Protein A Resin

  • Brand: Genscript
  • Product Code: L00210
  • Availability: Pre-Order
  • $0.00

  • Ex Tax: $0.00

Related Products

Protein G Resin

Protein G Resin

DescriptionGenScript Protein G Resin is an affinity chromatography medium designed for easy, one-ste..

$0.00 Ex Tax: $0.00

Empty Gravity Chromatography Columns

Empty Gravity Chromatography Columns

Features: Frits are optimized for minimizing non-specific adsorption Solvents can be fed with grav..

$0.00 Ex Tax: $0.00

Empty FPLC column

Empty FPLC column

The empty FPLC columns are HiTrap-like, ÄKTA-compatible, and have proven to be extremely handy,..

$0.00 Ex Tax: $0.00

Featured

SAFEVAC

SAFEVAC

DLAB SAFEVAC - Vacuum Aspiration SystemsSafeVac is designed for laboratory waste recovery ..

$0.00 Ex Tax: $0.00

EVE™ Plus |The World's Fastest Cell Counter

EVE™ Plus |The World's Fastest Cell Counter

The World's Fastest Automated Cell CounterAuto focus & Manual focusAuto focus total cell, l..

$0.00 Ex Tax: $0.00

JUPITER | Single & parallel autoclavable stirred mini-fermenter/bioreactor

JUPITER | Single & parallel autoclavable stirred mini-fermenter/bioreactor

The Jupiter platform offers multiple autoclavable vessel sizes and designs from 2.0 L up to 10.0 L t..

$0.00 Ex Tax: $0.00

CGQ - Cell Growth Quantifier

CGQ - Cell Growth Quantifier

The CGQ is an optical sensor-based technology for non-invasive online biomass monitoring in shake fl..

$0.00 Ex Tax: $0.00

WhatsApp Chat

Hi Guest! Please click one of our representatives Below To Chat On WhatsApp.

WhatsApp Chat