The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.The GenScript Ni-Charged MagBeads are average 40 μM in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6xHis-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).Total Volume: 2 ml settled Beads (8 ml 25% slurry) Key Features
- Quick and convenient separation accomplished by magnetic force
- High binding capacity with 5-20mg of 6xHis-tagged protein/ml
- Compatible with various reagents needed in the purification process (see table 1)
- Resin can be regenerated for multiple uses
- Extremely low nonspecific binding
Table 1 Reagents Compatible with Ni-charged Magnetic BeadsDenaturants | Detergents | Reducing agents | Salts | Others |
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6 M Gu•HCl | 2% Triton X-100 | 20 mM β-ME | 4 M MgCl2 | 50% glycerol |
8 M Urea | 2% Tween 20 | 1 mM DTT | 5 mM CaCl2 | 20% ethanol |
| 1% CHAPS | | 2 M NaCl | 1 mM EDTA |
StorageStore at 4℃, do NOT freeze.