The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.The GenScript Ni-Charged MagBeads are average 40 μM in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6xHis-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).Total Volume: 2 ml settled Beads (8 ml 25% slurry) Key Features

• Quick and convenient separation accomplished by magnetic force
• High binding capacity with 5-20mg of 6xHis-tagged protein/ml
• Compatible with various reagents needed in the purification process (see table 1)
• Resin can be regenerated for multiple uses
• Extremely low nonspecific binding

Table 1 Reagents Compatible with Ni-charged Magnetic Beads

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu•HCl	2% Triton X-100	20 mM β-ME	4 M MgCl2	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl2	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA

StorageStore at 4℃, do NOT freeze.