• Ni-charged MagBeads
The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.The GenScript Ni-Charged MagBeads are average 40 μM in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6xHis-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).Total Volume: 2 ml settled Beads (8 ml 25% slurry) Key Features
  • Quick and convenient separation accomplished by magnetic force
  • High binding capacity with 5-20mg of 6xHis-tagged protein/ml
  • Compatible with various reagents needed in the purification process (see table 1)
  • Resin can be regenerated for multiple uses
  • Extremely low nonspecific binding
Table 1 Reagents Compatible with Ni-charged Magnetic Beads
DenaturantsDetergentsReducing agentsSaltsOthers
6 M Gu•HCl2% Triton X-10020 mM β-ME4 M MgCl250% glycerol
8 M Urea2% Tween 201 mM DTT 5 mM CaCl220% ethanol
1% CHAPS 2 M NaCl1 mM EDTA
StorageStore at 4℃, do NOT freeze.

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Ni-charged MagBeads

  • Brand: Genscript
  • Product Code: L00295
  • Availability: Pre-Order
  • $0.00

  • Ex Tax: $0.00

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