DescriptionDYKDDDDK or FLAG® tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. GenScript Anti-DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate. Table 1 lists the main characteristics of Anti-DYKDDDDK G1 Affinity Resin. Key Features

• High DYKDDDDK or FLAG®-tag binding capacity and ultralow levels of non-specific binding
• Multiple elution methods for diverse protein characteristics and different requirements
• Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Characteristics:

Product content	50% settled resin in TBS with 0.02% sodium azide
Matrix	4% cross-linked agarose
Average bead size	90 μm
Ligand	Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity	Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin
Storage and stability	Store at 2-8 °C for up to 12 months. Do not freeze the resin.
Resin reuse	The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method	Acid, alkaline, neutral, peptide competitive and SDS-PAGE loading buffer elutions
Reagents compatibility	Compatible with commonly used reagents at certain concentrations

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