• Protein G Resin
DescriptionGenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. Protein G Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg goat IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume5 ml settled resin (10 ml 50% slurry)Storage:Store at 4°C, do NOT freeze. Key Features
  • Broad IgG binding spectrum
  • Binding specificity complements of protein A
  • Agarose media
  • No specific albumin binding
  • Optimized homogeneous recombinant ligand
  • High capacity
  • Available in bulk size
Specifications
LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitution Approximately 2 mg protein G/ml settled resin
Static binding capacity >20 mg goat IgG/ml settled resin
Matrix sphericalagarose, 4% cross-linked
Average particle size90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months if store unopened at 2-8℃

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Protein G Resin

  • Brand: Genscript
  • Product Code: L00209
  • Availability: Pre-Order
  • $0.00

  • Ex Tax: $0.00

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