The RevoSeizer® 100mL Ultrafiltration Cup is an advanced concentration tool engineered for large-volume biological samples ranging from 20 to 90mL. Built on the proven RevoSeizer® centrifugal ultrafiltration platform, it combines a vertical membrane design with a modified PES membrane material to deliver rapid concentration, high bioactivity retention, and minimal sample loss — making it the ideal solution for processing proteins, nucleic acids, viruses, and other sensitive biological macromolecules.
Product Description
The RevoSeizer® 100mL Ultrafiltration Cup is an iterative upgrade of the RevoSeizer® centrifugal ultrafiltration tube, specifically designed for large-volume biological sample processing (20–90mL). Its vertical membrane architecture combined with a modified PES (polyethersulfone) membrane achieves rapid concentration, preserved biological activity, and low sample loss — making it the preferred choice for protein, nucleic acid, and virus concentration workflows.
Key Features
- High-Efficiency Concentration: Modified PES ultrafiltration membrane with vertical membrane design enables 30–50× protein concentration. High flow rate with minimal protein damage, recovery rate >85%, and protein adsorption <10 μg/cm².
- Easy to Use: No tubing connections required. Concentrate 90mL samples in as little as 30 minutes.
- Over-Centrifugation Protection: Integrated locking volume design prevents over-centrifugation and protects sample integrity.
- Flexible MWCO Options: Available in multiple molecular weight cut-off (MWCO) ratings to accommodate diverse separation requirements.
Typical Applications
- Biopharmaceuticals: Virus concentration and isolation, crude separation of biomolecular mixtures and nanoparticles — accelerating drug development workflows.
- Protein Research: Buffer exchange, protein concentration and desalting, removal of nucleic acids or chromatography components — while preserving native protein activity.
- Molecular Biology: Removal of primers, adapters, or molecular markers from reaction mixtures to improve downstream experimental purity.
Product Structure
The ultrafiltration cup consists of three components: a lid, a filtration assembly, and a collection cup.
Technical Specifications
| Parameter | Detail |
|---|---|
| Filtration Assembly Material | PC (Polycarbonate) |
| Membrane Material | PES (Polyethersulfone) |
| Collection Cup Material | PC (Polycarbonate) |
| Lid Material | PP (Polypropylene) |
| Dimensions | Length: 123mm / Diameter: 62mm |
| Effective Membrane Area | 23 cm² |
| Sample Volume Range | 20mL – 90mL |
| Dead (Retentate) Volume | <220 μL |
| Lock-Stop Volume | 300–400 μL |
| Operating Temperature | 0–40°C |
| pH Range | 1–12 |
| Compatible Rotor | 250mL swing-bucket rotor |
| Maximum Centrifugal Force | 2,000 × g |
| Sterilization | Pre-use sterilization with 70% ethanol by device filtration |
| Storage (Unused) | Store at room temperature in a cool, dry place |
| Storage (After Use) | Keep moist; recommended to fill with 20% ethanol or purified water |
Ordering Information
| Catalog No. | Volume | MWCO | Packaging |
|---|---|---|---|
| UCPES100005N | 100mL | 5 kDa | 2 units/box |
| UCPES100010N | 100mL | 10 kDa | 2 units/box |
| UCPES100030N | 100mL | 30 kDa | 2 units/box |
| UCPES100050N | 100mL | 50 kDa | 2 units/box |
| UCPES100100N | 100mL | 100 kDa | 2 units/box |
RevoSeizer® 100mL Ultrafiltration Cup
- Brand: Membrane Solutions
- Product Code: UCPES100xxxN
- Availability: In Stock
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$0.00
- Ex Tax: $0.00
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