Premium PVDF membrane from Carl ROTH, suitable for direct fluorescence detection

ROTI®Fluoro PVDF is a white, microporous membrane made from naturally hydrophobic polyvinylidene fluoride. The membrane is of superior protein binding capacity, making it perfectly suited for all standard and special applications in the field of protein analysis.

ROTI®Fluoro PVDF has been specially optimised for direct detection via fluorescence.
ROTI®Fluoro PVDF binds proteins adhesively via hydrophobic interactions. It enables very sensitive protein detection by keeping the background very low, resulting in a good signal-to-noise ratio with all standard detection systems. The mechanically rigid membrane is of high tensile strength and tear-proof, and does not crack or curl during handling. Therefore, signals remain clear and distinct, and are undisturbed by false-positive signals caused by membranes crinkles.
Briefly immerse ROTI®Fluoro PVDF membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every aqueous transfer system.

The membrane ROTI®Fluoro PVDF is only conditionally suitable for use with chemiluminescence detection. The membranes are specially coated for fluorescence applications and this coating can reduce the sensitivity of chemiluminescence. For chemiluminescent assays we recommend the use of ROTI®PVDF membranes.

Western- and Far-Western-Blot, analysis of small peptides, Immunoblot, Dot-blot, binding assays, N-terminal sequencing
Detection systems: fluorescence, radioactivity, colour"