RevoSeizer® Centrifugal Filter (Ultrafiltration Tube)
The RevoSeizer® Centrifugal Filter is a high-performance concentration and purification device designed for biological sample processing. Available in 0.5mL, 4mL, and 15mL formats, its unique vertical dual-sided membrane design maximizes filtration area, enabling fast throughput and high sample recovery — typically greater than 90%. The modified PES membrane maintains a gentle concentration environment that preserves protein activity and conformation, while the built-in anti-dry locking mechanism prevents over-centrifugation and potential sample loss.
Key Features
- High Recovery Rate: Precise retention with low protein adsorption and >90% sample recovery
- Dual-Sided Vertical Membrane Design: Minimizes membrane clogging, reduces holdup volume, and maximizes centrifugal efficiency
- Excellent Biocompatibility: Minimal extractables; USP Class VI certified materials
- Anti-Dry Locking Design: Physical dead-stop prevents over-centrifugation, ensuring reliable and reproducible results
- Multiple MWCO Options: Available in 5, 10, 30, 50, and 100 kDa molecular weight cut-offs
- OEM Customization Available
Applications
Ultrafiltration:
- Protein and nucleic acid concentration and desalting
- Buffer exchange of chromatography fractions
- Harvesting biomolecules from culture media
- Virus concentration and isolation
- Coarse separation of biomolecular mixtures and nanoparticles
- Removal of debris and particles from cell lysates
Microfiltration:
- DNA isolation from agarose gels
- Protein, oligonucleotide, and RNA separation from polyacrylamide gels
- Sample clarification prior to HPLC analysis
- Cell media removal
- Biological sample filtration
- Collection and washing of processed particles or beads
- Packing chromatographic media for analytical or process development
Technical Specifications
| Parameter | 0.5mL | 4mL | 15mL |
|---|---|---|---|
| Filter Material | PC (Polycarbonate) | SBC (Styrene Butadiene Copolymer) | SBC |
| Membrane Material | PES (Polyethersulfone) | PES | PES |
| Filtrate Tube / Cap | Polypropylene | Polypropylene | Polypropylene |
| Effective Filter Area | 0.9 cm² | 3.5 cm² | 7.2 cm² |
| Retention Volume | ≤5 μL | ≤20 μL | ≤30 μL |
| Locking Volume | 10–20 μL | 50–100 μL | 300 μL |
| Operating Temperature | 0–40°C | 0–40°C | 0–40°C |
| pH Range | 1–14 | 1–14 | 1–14 |
| Max Centrifugal Force (UF) | 14,000 × g | 4,000 × g (swing) / 5,000 × g (fixed) | 4,000 × g (swing) / 5,000 × g (fixed) |
| Compatible Centrifuge Tube | 1.5mL conical | 15mL conical | 50mL conical |
| Sterilization | Non-sterile; can be disinfected with 70% ethanol by filtration |
RevoSeizer® Ultrafiltration Centrifuge Tubes
- Brand: Membrane Solutions
- Product Code: UTPESXX0XXN
- Availability: In Stock
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$0.00
- Ex Tax: $0.00
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